ACTN1 - Antibodies - The Human Protein Atlas

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ACTN1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA006035

Antibody CAB004303

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA006035

sc-17829

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

ACTN2 - ENSG00000077522 [83%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA006035

Antibody CAB004303

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006035

Antibody CAB004303

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.

Antibody dilution

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006035

Antibody CAB004303

Standard validation

Approved

Figure description

Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 74 cell types

Retrieval

HIER pH6

Antibody dilution

1:350

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006035

Antibody CAB004303

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

107.6, 107.1, 105.6, 104.9, 103.9, 103.2, 103.1, 102.7, 94.8, 29.6, 27.1

107.1, 105.6, 103.1, 102.7, 94.8, 34.3, 29.6, 28.8, 27.1, 24.3, 16.3, 14, 9.4, 8.2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA006035

Antibody CAB004303

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

ANTIGEN INFORMATION

Antibody HPA006035

Antibody CAB004303

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

121

Antigen sequence

SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYG
KLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSS
FYHAFSGAQKAETAANRICKV

Matching transcripts

ACTN1-001 - ENSP00000193403 [100%]
ACTN1-002 - ENSP00000414272 [100%]
ACTN1-005 - ENSP00000377941 [100%]
ACTN1-006 - ENSP00000439828 [100%]
ACTN1-007 - ENSP00000366035 [100%]
ACTN1-011 - ENSP00000450764 [100%]
ACTN1-012 - ENSP00000450903 [100%]

Other gene match

ACTN2 - ENSG00000077522 [83%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]

ANTIGEN VIEW

ACTN1-001	ACTN1-002	ACTN1-005	ACTN1-006	ACTN1-007	ACTN1-011	ACTN1-012

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.