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Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Antibody dilution
1:58
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040354
Antibody HPA044111
Antibody CAB034010
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate nuclear and cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate nucelar and cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 35 tissues Protein: detected in 66 cell types
RNA: detected in 35 tissues Protein: detected in 73 cell types
RNA: detected in 35 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:160
1:15
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040354
Antibody HPA044111
Antibody CAB034010
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
30.1, 23.6
30.1
30.1, 23.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA040354
Antibody HPA044111
Antibody CAB034010
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
