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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040809
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Expression
RNA: detected in 9 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040809
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402712)
