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THOC1

ANTIBODY INFORMATION
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019096   HPA019687  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

 1   1  

VALIDATION SUMMARY
 
 

Antibody HPA019096

  

Antibody HPA019687

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA019687.		  
Validated

Antibody staining overlaps with antibody HPA019096.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.  

Antibody dilution

 1:50   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 54 cell types		   RNA: detected in 36 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 75.7, 41.5   75.7, 31.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019096

  

Antibody HPA019687

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   127  

Antigen sequence

 
VFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNEQESTLGQKHT
EDREEGMDVEEGEMGDEEAPTTCSIPIDYNLYRKFWS
  
KILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENM
VENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKL
AKELPPPSEEIKTGEDEDEEDNDALLK
 

Matching transcripts

 THOC1-001 - ENSP00000261600 [100%]
THOC1-201 - ENSP00000480460 [100%]
THOC1-202 - ENSP00000481599 [100%]
  THOC1-001 - ENSP00000261600 [100%]
THOC1-201 - ENSP00000480460 [100%]
THOC1-020 - ENSP00000473276 [83%]
 

Other gene match

    
ANTIGEN VIEW
THOC1-001
THOC1-020
THOC1-201
THOC1-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA019096

  

Antibody HPA019687

  

1.

 Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers
Domínguez-Sánchez MS et al
BMC Cancer 2011;11:77
   Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers
Domínguez-Sánchez MS et al
BMC Cancer 2011;11:77
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.