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PGM1

ANTIBODY INFORMATION
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA024190   HPA024637   HPA046329   H00005236-M01  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

     PGM5 - ENSG00000154330 [100%]
    

Released in version

 5   5   12   2  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  
Immunocytochemistry


Validated
  

Validated
      
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA024637.		  
Validated

Antibody staining overlaps with antibody HPA024190.		      
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to actin filaments.   Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.      

Antibody dilution

 1:75   1:50      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 27 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 27 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:80   1:150   1:25   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.8, 61.4, 40.3   63.8, 61.4, 40.3   63.8, 62.2, 61.4, 41.8, 21.8   63.8, 61.4, 40.3  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024190

  

Antibody HPA024637

  

Antibody HPA046329

  

Antibody CAB004666

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 78   79   25    

Antigen sequence

 
GSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQD
PQVMLAPLISIALKVSQLQERTGRTAPT
  
SILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALM
FDRSFVGKQFSANDKVYTVEKADNFEYSD
  
AAGGIILTASHCPGGPGGEFGVKFN
  

											 
																				

																		

										
											
Matching transcripts

												
																					PGM1-001 - ENSP00000360125 [100%] 
PGM1-002 - ENSP00000360124 [100%] 
PGM1-201 - ENSP00000443449 [100%] 

											 
																						PGM1-001 - ENSP00000360125 [99%] 
PGM1-002 - ENSP00000360124 [99%] 
PGM1-201 - ENSP00000443449 [99%] 

											 
																						PGM1-001 - ENSP00000360125 [80%] 
PGM1-002 - ENSP00000360124 [80%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						PGM5 - ENSG00000154330 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PGM1-001
PGM1-002
PGM1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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