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ARG2

ANTIBODY INFORMATION
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA000663   sc-20151  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 1   4  

References

 3    

VALIDATION SUMMARY
 
 

Antibody HPA000663

  

Antibody CAB009435

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.  

Antibody dilution

   1:25  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.   Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 30 tissues
Protein: detected in 24 cell types		   RNA: detected in 30 tissues
Protein: detected in 27 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420091)		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.6   38.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA000663

  

Antibody CAB009435

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 147    

Antigen sequence

 
MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIR
EAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELA
EVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHAD
  

											 
																				

																		

										
											
Matching transcripts

												
																					ARG2-001 - ENSP00000261783 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ARG2-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA000663

												
										 
																				
											
											
											
Antibody CAB009435

												
										 
																		

																	

										
											
1.

												
																						Local arginase inhibition during early reperfusion mediates cardioprotection via increased nitric oxide production

	Gonon AT et al

	PLoS One 2012;7(7):e42038

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

	Grönros J et al

	Am J Physiol Heart Circ Physiol 2011;300(4):H1174-81

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Arginase inhibition improves endothelial function in patients with coronary artery disease and type 2 diabetes mellitus

	Shemyakin A et al

	Circulation 2012;126(25):2943-50

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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