KAT6A - Antibodies - The Human Protein Atlas

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KAT6A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Provider

Atlas Antibodies
Sigma-Aldrich

SDIX

Product name

HPA063266

HPA065052

2162

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.

Antibody dilution

1:97

1:125

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Standard validation

Supported

Figure description

Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

1:3000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

225, 84.1

225

225, 93.2, 84.1, 1.6

Antibody dilution

1:170

1:320

1:500

PROTEIN ARRAY

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2100

1:3900

ANTIGEN INFORMATION

Antibody HPA063266

Antibody HPA065052

Antibody CAB017023

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

SSAPQEQYGECGEKSEATQEQYTESEEQLVASEEQPSQDGKPDLPKRRLS
EGVEPWRGQLKKSPEALKCRLTEGSE

KELEEQPTREDVKEEPGVQESFLDANMQKSREKIKDKEETELDSEEEQPS
HDTSVVSEQMAGSEDDHEEDSHTKE

Matching transcripts

KAT6A-001 - ENSP00000265713 [100%]
KAT6A-003 - ENSP00000399638 [100%]
KAT6A-004 - ENSP00000385888 [100%]
KAT6A-201 - ENSP00000380136 [100%]

KAT6A-001 - ENSP00000265713 [100%]
KAT6A-004 - ENSP00000385888 [100%]
KAT6A-201 - ENSP00000380136 [100%]

Other gene match

ANTIGEN VIEW

KAT6A-001	KAT6A-003	KAT6A-004	KAT6A-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.