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Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
Antibody dilution
1:88
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA004820
Antibody HPA062839
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Expression
RNA: detected in 25 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
Antibody dilution
1:350
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004820
Antibody HPA062839
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411012)
Target mass (kDa)
71.1, 69.3
71.1, 69.3
Antibody dilution
1:250
1:290
PROTEIN ARRAY
Antibody HPA004820
Antibody HPA062839
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
