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WBP11

ANTIBODY INFORMATION
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039710   HPA040037   HPA046403   HPA049126  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 14   8   12   12  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  
Immunocytochemistry

Supported       Supported  
Immunohistochemistry

  Approved   Approved   Approved  
Western blot

Supported   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Approved   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Standard validation

 Supported       Supported  

       

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.       Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:30       1:139  

Literature conformity

 The subcellular location is supported by literature.       The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Standard validation

   Approved   Approved   Approved  

       

Figure description

   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong nuclear and cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human heart muscle shows moderate nuclear positivity in myocytes.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

   1:3500   1:100   1:350  

Literature conformity

   Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 70   70   70   70  

Antibody dilution

 1:250   1:250   1:250   1:700  

PROTEIN ARRAY
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:30000   1:6000   1:5600  

ANTIGEN INFORMATION
 
 

Antibody HPA039710

  

Antibody HPA040037

  

Antibody HPA046403

  

Antibody HPA049126

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 93   97   42   26  

Antigen sequence

 
DIYKELRKLEVEYEQKRAQLSQYFDAVKNAQHVEVESIPLPDMPHAPSNI
LIQDIPLPGAQPPSILKKTSAYGPPTRAVSILPLLGHGVPRLP
  
MGRRSTSSTKSGKFMNPTDQARKEARKRELKKNKKQRMMVRAAVLKMKDP
KQIIRDMEKLDEMEFNPVQQPQLNEKVLKDKRKKLRETFERILRLYE
  
QRKSEDNSAVPLAKAAPKSGPSVPVSVQTKDDVYEDFMKEME
  
KPQITNPKAEITRFVPTALRVRRENK
 

Matching transcripts

 WBP11-001 - ENSP00000261167 [100%]
  WBP11-001 - ENSP00000261167 [100%]
  WBP11-001 - ENSP00000261167 [95%]
  WBP11-001 - ENSP00000261167 [100%]
 

Other gene match

        
ANTIGEN VIEW
WBP11-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.