PREP - Antibodies - The Human Protein Atlas

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PREP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA031388

HPA031389

HPA031390

AF4308

Host species

Rabbit

Goat

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:179

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA031390 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA031388 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in granular pattern in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 78 cell types

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 58 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:60

1:1000

1:25

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

80.7

80.7, 5.1

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA031388

Antibody HPA031389

Antibody HPA031390

Antibody CAB025414

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

100

Antigen sequence

IACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKK
DTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDY

LVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIV
GRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP

LSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGT
VFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKD

Matching transcripts

PREP-001 - ENSP00000358106 [100%]

Other gene match

ANTIGEN VIEW

PREP-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.