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FOLH1

ANTIBODY INFORMATION
 
 

Antibody HPA010593

  

Antibody CAB001451

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA010593   NCL-L-PSMA  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 3   1  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA010593

  

Antibody CAB001451

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010593

  

Antibody CAB001451

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human prostate shows distinct cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 8 cell types		   RNA: detected in 31 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010593

  

Antibody CAB001451

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 84.3, 82.5, 80.6, 78.8   84.3, 82.5, 80.6, 78.8, 50.1, 7.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010593

  

Antibody CAB001451

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA010593

  

Antibody CAB001451

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 106    

Antigen sequence

 
ADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRR
GIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGP
GFTGNF
  

											 
																				

																		

										
											
Matching transcripts

												
																					FOLH1-001 - ENSP00000256999 [100%] 
FOLH1-002 - ENSP00000349129 [100%] 
FOLH1-003 - ENSP00000344131 [100%] 
FOLH1-004 - ENSP00000431463 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FOLH1-001
FOLH1-002
FOLH1-003
FOLH1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA010593

												
										 
																				
											
											
											
Antibody CAB001451

												
										 
																		

																	

										
											
1.

												
																						GAD1 is a biomarker for benign and malignant prostatic tissue

	Jaraj SJ et al

	Scand J Urol Nephrol 2011;45(1):39-45

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes

	Hudson EP et al

	Sci Rep 2012;2:706

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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