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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Antibody dilution
1:31
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:30
1:25
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002548
Antibody CAB022718
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002548
Antibody CAB022718
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
481.9, 481
481.9, 481, 133.2, 23.9, 10.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002548
Antibody CAB022718
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
