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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
Antibody dilution
1:20
1:75
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018122
Antibody HPA027478
Antibody HPA028386
Antibody CAB010337
Standard validation
Uncertain
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Immunohistochemical staining of human colon shows distinct positivity in microvilli of glandular cells.
Immunohistochemical staining of human fallopian tube shows distinct positivity in cilia.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 7 cell types
RNA: detected in 37 tissues Protein: detected in 47 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:2500
1:35
1:10
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018122
Antibody HPA027478
Antibody HPA028386
Antibody CAB010337
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402577)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
