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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Antibody dilution
1:52
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039927
Antibody HPA040857
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human esophagus shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 50 cell types
RNA: detected in 37 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:30
1:225
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039927
Antibody HPA040857
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415374)
Target mass (kDa)
40.2
40.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039927
Antibody HPA040857
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
