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Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:54
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049850
Antibody HPA053524
Antibody CAB009187
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA053524 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA049850 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 51 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:75
1:115
1:400
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049850
Antibody HPA053524
Antibody CAB009187
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56.5, 40.5
56.5, 40.5
56.5, 40.5
Antibody dilution
1:150
1:80
1:500
PROTEIN ARRAY
Antibody HPA049850
Antibody HPA053524
Antibody CAB009187
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
