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Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Antibody dilution
1:222
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA000634
Antibody HPA001004
Antibody CAB034139
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human bronchus shows cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 67 cell types
RNA: detected in 37 tissues Protein: detected in 45 cell types
RNA: detected in 37 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:250
1:25
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000634
Antibody HPA001004
Antibody CAB034139
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400594)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.2
39.2
39.2
Antibody dilution
1:500
1:250
1:500
PROTEIN ARRAY
Antibody HPA000634
Antibody HPA001004
Antibody CAB034139
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
