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Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge.
Antibody dilution
1:13
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002830
Antibody CAB004270
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in a subset of bone marrow poietic cells.
Expression
RNA: detected in 23 tissues Protein: detected in 61 cell types
RNA: detected in 23 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:750
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002830
Antibody CAB004270
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400469)
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Testis
Target mass (kDa)
18.6, 16.4
18.6, 16.4, 15.6, 13.4, 9, 8.5
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002830
Antibody CAB004270
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
