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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Antibody dilution
1:38
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002329
Antibody HPA075090
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows distinct nuclear positivity in seminiferus duct cells.
Expression
RNA: detected in 13 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002329
Antibody HPA075090
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402958)
Target mass (kDa)
27.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002329
Antibody HPA075090
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
