Cell atlas - ZBTB25 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	ZBTB25
Gene description	Zinc finger and BTB domain containing 25
Protein class	Plasma proteins Predicted intracellular proteins Transcription factors
Predicted localization	Intracellular
Number of transcripts	4
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.
Mouse	Localized to the Nucleoplasm in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 1 antibody. HPA054699
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA054699	A-431	Nucleoplasm
HPA054699	HeLa	Nucleoplasm
HPA054699	U-2 OS	Nucleoplasm

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA054699

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 2

RFI

66%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA054699	siRNA 2	U-2 OS
		HPA054699	scrambled	U-2 OS

MOUSE CELLS

Main location

Nucleoplasm

Additional location

Vesicles

Mouse gene

ENSMUSG00000056459 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA054699	NIH 3T3	Nucleoplasm
	HPA054699	NIH 3T3	Vesicles

GENE INFORMATION
Gene name	ZBTB25 (HGNC Symbol)
Synonyms	C14orf51, KUP, ZNF46
Description	Zinc finger and BTB domain containing 25 (HGNC Symbol)
Entrez gene summary
Chromosome	14
Cytoband	q23.3
Chromosome location (bp)	64449106 - 64505213
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000089775 (version 83.38)
Entrez gene	7597
UniProt	P24278 (UniProt - Evidence at protein level)
neXtProt	NX_P24278
Antibodypedia	ZBTB25 antibodies

PROTEIN BROWSER

ZBTB25-001	ZBTB25-002	ZBTB25-003	ZBTB25-004

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ZBTB25-001 ENSP00000476746 ENST00000608382	P24278 [Direct mapping] Zinc finger and BTB domain-containing protein 25 Show all	Predicted intracellular proteins Plasma proteins Transcription factors Zinc-coordinating DNA-binding domains Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000977 [RNA polymerase II regulatory region sequence-specific DNA binding] GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding] GO:0003677 [DNA binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0006351 [transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0010467 [gene expression] GO:0046872 [metal ion binding] Show all	435 aa 49 kDa	No	0
ZBTB25-002 ENSP00000378204 ENST00000394715	P24278 [Direct mapping] Zinc finger and BTB domain-containing protein 25 Show all	Predicted intracellular proteins Plasma proteins Transcription factors Zinc-coordinating DNA-binding domains Protein evidence (Ezkurdia et al 2014) Show all	GO:0000977 [RNA polymerase II regulatory region sequence-specific DNA binding] GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding] GO:0003677 [DNA binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0006351 [transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0010467 [gene expression] GO:0046872 [metal ion binding] Show all	435 aa 49 kDa	No	0
ZBTB25-003 ENSP00000450718 ENST00000555220	G3V2K3 [Direct mapping] Zinc finger and BTB domain-containing protein 25 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000977 [RNA polymerase II regulatory region sequence-specific DNA binding] GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0006357 [regulation of transcription from RNA polymerase II promoter] Show all	86 aa 9.6 kDa	No	0
ZBTB25-004 ENSP00000451046 ENST00000555424	G3V351 [Direct mapping] Zinc finger and BTB domain containing 25, isoform CRA_b; Zinc finger and BTB domain-containing protein 25 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000977 [RNA polymerase II regulatory region sequence-specific DNA binding] GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0046872 [metal ion binding] Show all	94 aa 10.9 kDa	No	0