Cell atlas - NECAP1 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	NECAP1
Gene description	NECAP endocytosis associated 1
Protein class	Disease related genes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to vesicles. In addition localized to the cytosol.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Vesicles (approved)
Additional location	In addition localized to the Cytosol (approved)

DATA RELIABILITY
Reliability score	Approved based on 1 antibody. HPA053980
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to vesicles. In addition localized to the cytosol.

Main location

Vesicles (approved)

Additional location

Cytosol (approved)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA053980	HeLa	Vesicles
HPA053980	MCF7	Cytosol
HPA053980	U-2 OS	Vesicles

MOUSE CELLS

Main location

Not available

Mouse gene

ENSMUSG00000030327 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA053980	NIH 3T3	No staining

GENE INFORMATION
Gene name	NECAP1 (HGNC Symbol)
Synonyms	DKFZP566B183
Description	NECAP endocytosis associated 1 (HGNC Symbol)
Entrez gene summary	This gene encodes a protein containing two characteristic WXXF motifs. The encoded protein localizes to clathrin-coated vesicles, where it binds components of the adapter protein complexes and aids in endocytosis. Loss of function of this gene results in early infantile epileptic encephalopathy-21. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Chromosome	12
Cytoband	p13.31
Chromosome location (bp)	8082211 - 8097771
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000089818 (version 83.38)
Entrez gene	25977
UniProt	Q8NC96 (UniProt - Evidence at protein level)
neXtProt	NX_Q8NC96
Antibodypedia	NECAP1 antibodies

PROTEIN BROWSER

NECAP1-001	NECAP1-010

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
NECAP1-001 ENSP00000341737 ENST00000339754	Q8NC96 [Direct mapping] Adaptin ear-binding coat-associated protein 1 Show all	Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005886 [plasma membrane] GO:0005905 [coated pit] GO:0006897 [endocytosis] GO:0015031 [protein transport] GO:0016020 [membrane] GO:0030125 [clathrin vesicle coat] Show all	275 aa 29.7 kDa	No	0
NECAP1-010 ENSP00000439319 ENST00000540083	F5H2U7 [Direct mapping] Adaptin ear-binding coat-associated protein 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		117 aa 11.9 kDa	No	0