ARHGAP4 - Antibodies - The Human Protein Atlas

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ARHGAP4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001012

Antibody HPA001083

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA001012

HPA001083

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001012

Antibody HPA001083

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA001012

Antibody HPA001083

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & focal adhesion sites.

Antibody dilution

1:5

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA001012

Antibody HPA001083

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA001083 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA001012 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA001083 across 70 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA001012 across 70 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp.

Expression

RNA: detected in 36 tissues
Protein: detected in 27 cell types

RNA: detected in 36 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

Antibody dilution

1:350

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001012

Antibody HPA001083

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

109.4, 105, 102.6, 85.4

Antibody dilution

1:500

PROTEIN ARRAY

Antibody HPA001012

Antibody HPA001083

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA001012

Antibody HPA001083

Antigen

Recombinant protein fragment

Length (aa)

114

115

Antigen sequence

EHNGMRGLIPHKYITLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHR
PEPCTSPEAMGPSGHRRRCLVPASPEQHVEVDKAVAQNMDSVFKELLGKT
SVRQGLGPASTTSP

DRQTIETEEVNKTLKATLQALLEVVASDDGDVLDSFQTSPSTESLKSTSS
DPGSRQAGRRRGQQQETETFYLTKLQEYLSGRSILAKLQAKHEKLQEALQ
RGDKEEQEVSWTQYT

Matching transcripts

ARHGAP4-001 - ENSP00000359045 [100%]
ARHGAP4-003 - ENSP00000203786 [100%]
ARHGAP4-011 - ENSP00000359033 [100%]
ARHGAP4-024 - ENSP00000377322 [100%]

Other gene match

ANTIGEN VIEW

ARHGAP4-001	ARHGAP4-003	ARHGAP4-011	ARHGAP4-024

RELEVANT PUBLICATIONS

Antibody HPA001012

Antibody HPA001083

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.