MAEA - Antibodies - The Human Protein Atlas

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MAEA

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036886

Antibody HPA058185

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036886

HPA058185

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036886

Antibody HPA058185

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036886

Antibody HPA058185

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:21

1:182

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036886

Antibody HPA058185

Standard validation

Approved

Figure description

Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:750

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036886

Antibody HPA058185

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.4, 45.3, 45.1, 40.4, 39.8

49.4, 45.3, 45.1, 40.4, 39.8, 36.2, 30.4, 12.9

Antibody dilution

1:250

1:610

PROTEIN ARRAY

Antibody HPA036886

Antibody HPA058185

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:7250

ANTIGEN INFORMATION

Antibody HPA036886

Antibody HPA058185

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

YDNYRLHQLGNNSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSL
NKLAQPLPMAHCANSRLVCKISGDVMNENNPPMMLPNGY

GVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAASVWKR
KRMDRMMVEHLLRCGYYNTAVKL

Matching transcripts

MAEA-001 - ENSP00000302830 [100%]
MAEA-002 - ENSP00000422215 [100%]
MAEA-003 - ENSP00000264750 [100%]
MAEA-006 - ENSP00000426807 [100%]
MAEA-007 - ENSP00000424436 [100%]

MAEA-001 - ENSP00000302830 [100%]
MAEA-002 - ENSP00000422215 [100%]
MAEA-003 - ENSP00000264750 [100%]
MAEA-004 - ENSP00000427512 [100%]
MAEA-006 - ENSP00000426807 [100%]
MAEA-007 - ENSP00000424436 [100%]
MAEA-016 - ENSP00000427460 [100%]
MAEA-017 - ENSP00000421644 [100%]

Other gene match

ANTIGEN VIEW

MAEA-001	MAEA-002	MAEA-003	MAEA-004	MAEA-006	MAEA-007	MAEA-016	MAEA-017

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.