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RBM27

ANTIBODY INFORMATION
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035944   HPA036422  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 RP11-449H3.3 - ENSG00000275740 [100%]
   RP11-449H3.3 - ENSG00000275740 [100%]
  

Released in version

 7   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035944

  

Antibody HPA036422

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.  

Antibody dilution

   1:53  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human duodenum shows strong nuclear and membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:18   1:60  

Literature conformity

 Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 118.7, 114.5   118.7, 114.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA035944

  

Antibody HPA036422

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   87  

Antigen sequence

 
LLLQQQQTLSHLSQQHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQ
TSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSGGAGEDCQIFS
  
YTKNYLPLLEPVKPEPKPLVQEKEEIKEEVFQEPAEEERDGRKKKYPSPQ
KTRSESSERRTREKKREDGKWRDYDRYYERNELYREK
 

Matching transcripts

 RBM27-001 - ENSP00000265271 [100%]
  RBM27-001 - ENSP00000265271 [100%]
 

Other gene match

 RP11-449H3.3 - ENSG00000275740 [100%]
   RP11-449H3.3 - ENSG00000275740 [100%]
  
ANTIGEN VIEW
RBM27-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.