Fields »
ABOUT
HELP
BLOG
APOH

ANTIBODY INFORMATION
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA001654   HPA003732   MAB1066  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   2   5  

References

 3   1    

VALIDATION SUMMARY
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  
Immunocytochemistry

Approved      
Immunohistochemistry


Validated
   Supported  

Validated
  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.      

Antibody dilution

 1:39      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

    
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity, with a granular pattern, in hepatocytes.   Immunohistochemical staining of human kidney shows extra-cellular positivity in blood vessels and strong granular cytoplasmic positivity in cells of renal tubules.   Immunohistochemical staining of human lung shows distinct positivity in blood.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 30 cell types		   RNA: detected in 12 tissues
Protein: detected in 26 cell types		   RNA: detected in 12 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:250   1:125  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424960)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424960)		    

Target mass (kDa)

 38.3   38.3, 22.2, 19.9   38.3, 22.2, 19.9, 11.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:5000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001654

  

Antibody HPA003732

  

Antibody CAB022214

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 145   147    

Antigen sequence

 
ECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIEC
TKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSF
FCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFSKTDASDVK
  
EPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILE
NGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPII
CPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITC
  

											 
																				

																		

										
											
Matching transcripts

												
																					APOH-001 - ENSP00000205948 [99%] 

											 
																						APOH-001 - ENSP00000205948 [100%] 
APOH-003 - ENSP00000464301 [95%] 
APOH-002 - ENSP00000463553 [87%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
APOH-001
APOH-002
APOH-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001654

												
										 
																				
											
											
											
Antibody HPA003732

												
										 
																				
											
											
											
Antibody CAB022214

												
										 
																		

																	

										
											
1.

												
																						Classification of protein profiles from antibody microarrays using heat and detergent treatment

	Häggmark A et al

	N Biotechnol 2012;29(5):564-70

			
											 
																							Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						β2-Glycoprotein I/HLA class II complexes are novel autoantigens in antiphospholipid syndrome

	Tanimura K et al

	Blood 2015;125(18):2835-44

			Application: FC, WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.