HAUS4 - Antibodies - The Human Protein Atlas

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HAUS4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA029803

Antibody HPA071195

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA029803

HPA071195

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

RP11-298I3.5 - ENSG00000259132 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA029803

Antibody HPA071195

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA029803

Antibody HPA071195

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.

Antibody dilution

1:30

1:4

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA029803

Antibody HPA071195

Standard validation

Approved

Figure description

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:600

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA029803

Antibody HPA071195

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

42.4, 37.2, 28.1, 27.8, 22.6

42.4, 32.2, 21.7, 18.4

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA029803

Antibody HPA071195

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA029803

Antibody HPA071195

Antigen

Recombinant protein fragment

Length (aa)

124

145

Antigen sequence

RCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCGAMILKLRMEELKILSD
TYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEEFDRLVK
EYTVLKQATENKRWALQEFSKVYR

KLLLNLSQHVDESGLSLTLAKEQAQAWKEVRLHKTTWLRSEILHRVIQEL
LVDYYVKIQDTNVTSEDKKFHETLEQRLLVTELMRLLGPSQEREIPPLLG
LEKADLLELMPLSEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPN

Matching transcripts

HAUS4-001 - ENSP00000452260 [100%]
HAUS4-002 - ENSP00000452239 [100%]
HAUS4-003 - ENSP00000206474 [100%]
HAUS4-004 - ENSP00000343473 [100%]
HAUS4-005 - ENSP00000342776 [100%]
HAUS4-006 - ENSP00000451018 [100%]
HAUS4-201 - ENSP00000441026 [100%]

HAUS4-003 - ENSP00000206474 [100%]
HAUS4-020 - ENSP00000452261 [100%]
HAUS4-021 - ENSP00000452165 [100%]
HAUS4-201 - ENSP00000441026 [100%]
HAUS4-012 - ENSP00000452058 [82%]

Other gene match

RP11-298I3.5 - ENSG00000259132 [100%]

ANTIGEN VIEW

HAUS4-001	HAUS4-002	HAUS4-003	HAUS4-004	HAUS4-005	HAUS4-006	HAUS4-012	HAUS4-020	HAUS4-021	HAUS4-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.