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EZR

ANTIBODY INFORMATION
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
   NCI-CPTC
  

Product name

 HPA021616   3145   CPTC-Ezrin-1  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 5   2   9  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  
Immunocytochemistry

Supported   Supported   Supported  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.  

Antibody dilution

 1:112   1:50   1:2000  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach (upper) shows strong cytoplasmic positivity in parietal cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.   Immunohistochemical staining of human stomach (upper) shows distinct cytoplasmic positivity in parietal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:2500   1:200   1:40000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69.4   69.4   69.4  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA021616

  

Antibody CAB004035

  

Antibody CAB047324

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 123      

Antigen sequence

 
REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTK
KAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVD
QIKSQEQLAAELAEYTAKIALLE
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					EZR-001 - ENSP00000356042 [100%] 
EZR-002 - ENSP00000338934 [100%] 
EZR-201 - ENSP00000376016 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EZR-001
EZR-002
EZR-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA021616

												
										 
																				
											
											
											
Antibody CAB004035

												
										 
																				
											
											
											
Antibody CAB047324

												
										 
																		

																	

										
											
1.

												
																						Reduced expression of ezrin in urothelial bladder cancer signifies more advanced tumours and an impaired survival: validatory study of two independent patient cohorts

	Andersson G et al

	BMC Urol 2014;14:36

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Incident urothelial cancer in the Malmö Diet and Cancer Study: cohort characteristics and further validation of ezrin as a prognostic biomarker

	Wennersten C et al

	Diagn Pathol 2014;9:189

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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