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Antibody staining overlaps with antibody HPA065931.
Validated
Antibody staining overlaps with antibody HPA065868.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
Antibody dilution
1:159
1:164
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA065868
Antibody HPA065931
Antibody CAB046463
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:3000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA065868
Antibody HPA065931
Antibody CAB046463
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.9, 23.3, 23.2, 16.9
31.9, 23.3, 23.2, 18.9, 18.8
31.9, 23.3, 23.2, 18.9, 18.8, 16.9, 6.3
Antibody dilution
1:530
1:550
1:500
PROTEIN ARRAY
Antibody HPA065868
Antibody HPA065931
Antibody CAB046463
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
