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HIRA

ANTIBODY INFORMATION
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   University of Glasgow
   University of Glasgow
  

Product name

 HPA050714   HPA052902   n/a   001  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Supernatant  

Other gene match

        

Released in version

 13   13   7   11  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  
Immunocytochemistry

  Supported   Supported    
Immunohistochemistry

Supported     Supported   Supported  
Western blot

Uncertain   Uncertain   Supported   Supported  
Protein array

Supported   Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Standard validation

   Supported   Supported    

       

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

   1:7   1:13    

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human kidney shows strong nucleolar positivity in cells in tubules.     Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		     RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:80     1:50   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 111.8   111.8, 89.3   111.8, 89.3   111.8, 89.3  

Antibody dilution

 1:120   1:80   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:550      

ANTIGEN INFORMATION
 
 

Antibody HPA050714

  

Antibody HPA052902

  

Antibody CAB039243

  

Antibody CAB039244

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 102   119      

Antigen sequence

 
LKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEW
ETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTL
HC
  
TWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAAR
LFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFE
YRLREICKDLLGPVHYSTG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					HIRA-001 - ENSP00000263208 [100%] 

											 
																						HIRA-001 - ENSP00000263208 [100%] 
HIRA-002 - ENSP00000345350 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HIRA-001
HIRA-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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