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ANTIBODY INFORMATION
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA061615   1707-1  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

    

Released in version

 13   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA061615

  

Antibody CAB007786

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.    

Antibody dilution

 1:39    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 13 tissues
Protein: detected in 12 cell types		   RNA: detected in 13 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:25  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 111.8   111.8  

Antibody dilution

 1:130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1600    

ANTIGEN INFORMATION
 
 

Antibody HPA061615

  

Antibody CAB007786

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 64    

Antigen sequence

 
ECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEE
QTEETQVTEEVTEE
   

Matching transcripts

 NEFH-001 - ENSP00000311997 [100%]
   

Other gene match

    
ANTIGEN VIEW
NEFH-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.