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HMOX1

ANTIBODY INFORMATION
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Enzo Life Sciences
  

Product name

 HPA000635   ADI-OSA-110  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 1   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA000635

  

Antibody CAB017444

  
Immunocytochemistry

Uncertain    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.    

Antibody dilution

 1:10    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human bone marrow shows strong positivity in macrophages.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 32 cell types		   RNA: detected in 37 tissues
Protein: detected in 14 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:450  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400777)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 32.8, 21.6   32.8, 21.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA000635

  

Antibody CAB017444

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 197    

Antigen sequence

 
QDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVA
LEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPA
MQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSS
GEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAF
  

											 
																				

																		

										
											
Matching transcripts

												
																					HMOX1-001 - ENSP00000216117 [100%] 
HMOX1-002 - ENSP00000413316 [90%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HMOX1-001
HMOX1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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