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APOL1

ANTIBODY INFORMATION
 
 

Antibody HPA018885

  

Antibody CAB056156

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA018885   AMAb90530  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   10  

References

 4    

VALIDATION SUMMARY
 
 

Antibody HPA018885

  

Antibody CAB056156

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018885

  

Antibody CAB056156

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.   Immunohistochemical staining of human colon shows distinct positivity in plasma.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 9 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:3500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA018885

  

Antibody CAB056156

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.9, 44, 42.2   45.9, 44, 42.2, 18.1, 15, 13  

Antibody dilution

 1:250   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA018885

  

Antibody CAB056156

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018885

  

Antibody CAB056156

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 125    

Antigen sequence

 
SNFLSLAGNTYQLTRGIGKDIRALRRARANLQSVPHASASRPRVTEPISA
ESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVVYLVYESKHLHEG
AKSETAEELKKVAQELEEKLNILNN
   

Matching transcripts

 APOL1-001 - ENSP00000380448 [100%]
APOL1-003 - ENSP00000317674 [100%]
APOL1-201 - ENSP00000380449 [100%]
APOL1-202 - ENSP00000411507 [100%]
APOL1-203 - ENSP00000388477 [100%]
   

Other gene match

    
ANTIGEN VIEW
APOL1-001
APOL1-003
APOL1-201
APOL1-202
APOL1-203

RELEVANT PUBLICATIONS
 
 

Antibody HPA018885

  

Antibody CAB056156

  

1.

 APOL1 localization in normal kidney and nondiabetic kidney disease
Madhavan SM et al
J Am Soc Nephrol 2011;22(11):2119-28
    

2.

 APOL1 null alleles from a rural village in India do not correlate with glomerulosclerosis
Johnstone DB et al
PLoS One 2012;7(12):e51546
    

3.

 Exon 4-encoded sequence is a major determinant of cytotoxicity of apolipoprotein L1
Khatua AK et al
Am J Physiol Cell Physiol 2015;309(1):C22-37
Application: WB 		   

4.

 BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins
Heneghan JF et al
Am J Physiol Cell Physiol 2015;309(5):C332-47
Application: WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.