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Immunofluorescent staining of human cell line U-2 OS shows localization to peroxisomes.
Antibody dilution
1:57
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA052708
Antibody HPA060972
Standard validation
Approved
Figure description
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic and membranous positivity in smooth muscle cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
Antibody dilution
1:60
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052708
Antibody HPA060972
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
34.6, 10.5
34.6, 26.3, 25.7
Antibody dilution
1:80
1:190
PROTEIN ARRAY
Antibody HPA052708
Antibody HPA060972
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
