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PHF5A

ANTIBODY INFORMATION
 
 

Antibody HPA028885

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028885  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA028885

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Supported  
Western blot


Validated
  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028885

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:23  
 

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:20  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028885

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human duodenum shows nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:10  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028885

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 12.4  

Loading control

 Total protein image  

Antibody dilution

 1:58  
 

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409947)		  

Target mass (kDa)

 12.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA028885

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA028885

  

Antigen

 Recombinant protein fragment  

Length (aa)

 102  

Antigen sequence

 
AKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNY
GSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYE
RK
 

Matching transcripts

 PHF5A-001 - ENSP00000216252 [100%]
 

Other gene match

  
ANTIGEN VIEW
PHF5A-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA028885

  

1.

 An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses
Schreiber CA et al
PLoS Pathog 2015;11(8):e1005082
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.