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ANTIBODY INFORMATION
 
 

Antibody HPA047737

  

Antibody CAB000110

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA047737   MS-1835  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 12   1  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047737

  

Antibody CAB000110

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047737

  

Antibody CAB000110

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human spleen shows strong positivity in subsets of cells in red pulp.  

Expression

 RNA: detected in 30 tissues
Protein: detected in 4 cell types		   RNA: detected in 30 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH9  

Antibody dilution

 1:20   1:40  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047737

  

Antibody CAB000110

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 28.8   28.8  

Antibody dilution

 1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047737

  

Antibody CAB000110

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:800    

ANTIGEN INFORMATION
 
 

Antibody HPA047737

  

Antibody CAB000110

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 51    

Antigen sequence

 
GRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAF
K
  

											 
																				

																		

										
											
Matching transcripts

												
																					CTSG-001 - ENSP00000216336 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTSG-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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