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VTI1B

ANTIBODY INFORMATION
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044121   HPA048150   HPA060118  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   13   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Approved     Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.   Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.    

Antibody dilution

 1:109   1:48    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.     Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 46 cell types		     RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:1000     1:300  

Literature conformity

 Consistent with gene/protein characterization data.     Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 26.7   26.7   26.7  

Antibody dilution

 1:250   1:240   1:360  

PROTEIN ARRAY
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:1900   1:4250  

ANTIGEN INFORMATION
 
 

Antibody HPA044121

  

Antibody HPA048150

  

Antibody HPA060118

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 102   96   96  

Antigen sequence

 
ASSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEA
NETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPG
GR
  
RGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATET
DQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKV
  
RGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATET
DQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKV
 

Matching transcripts

 VTI1B-001 - ENSP00000450731 [100%]
  VTI1B-001 - ENSP00000450731 [100%]
  VTI1B-001 - ENSP00000450731 [100%]
 

Other gene match

      
ANTIGEN VIEW
VTI1B-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.