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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane & nucleoplasm and GFP expression in plasma membrane & nucleoplasm.
Antibody dilution
1:90
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:90
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA000907
Antibody HPA001275
Antibody CAB017442
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
RNA: detected in 37 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000907
Antibody HPA001275
Antibody CAB017442
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.6, 92.8, 92.7, 86.1
95.6, 92.8, 92.7, 86.1
95.6, 92.8, 92.7, 86.1, 82.7
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA000907
Antibody HPA001275
Antibody CAB017442
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
