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MTHFD1

ANTIBODY INFORMATION
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000704   HPA001290   HPA015006   HPA050052  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

     MTHFD1L - ENSG00000120254 [80%]
   MTHFD1L - ENSG00000120254 [96%]
  

Released in version

 1   4   4   13  

References

 1   1      

VALIDATION SUMMARY
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  
Immunocytochemistry


Validated
  

Validated
      
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot


Validated
   Supported   Supported   Supported  
Protein array

Supported   Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA001290.		  
Validated

Antibody staining overlaps with antibody HPA000704.		      
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.      

Antibody dilution

 1:115   1:250      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:2000   1:250   1:55  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		        

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		        

Target mass (kDa)

 110.6, 101.5, 28.2        

Loading control

 Total protein image        

Antibody dilution

 1:255        
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 110.6, 101.5, 28.2   110.6, 101.5   110.6, 105.9, 105.8, 101.5, 99.3   105.9, 105.8, 101.5, 99.3, 11.5, 10, 6.2  

Antibody dilution

 1:500   1:500   1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:2000   1:3000   1:1950  

ANTIGEN INFORMATION
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 142   156   145   25  

Antigen sequence

 
LNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVK
LKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSE
NSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCT
  
ILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDE
AKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNL
NLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSAL
EGAPHR
  
AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRL
GIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAP
TEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSK
  
FILPISDVRASIGAGFIYPLVGTVS
 

Matching transcripts

 MTHFD1-001 - ENSP00000450560 [99%]
MTHFD1-002 - ENSP00000438588 [99%]
MTHFD1-019 - ENSP00000216605 [99%]
  MTHFD1-002 - ENSP00000438588 [98%]
MTHFD1-019 - ENSP00000216605 [98%]
  MTHFD1-002 - ENSP00000438588 [100%]
MTHFD1-019 - ENSP00000216605 [100%]
  MTHFD1-014 - ENSP00000477199 [84%]
MTHFD1-019 - ENSP00000216605 [80%]
 

Other gene match

     MTHFD1L - ENSG00000120254 [80%]
   MTHFD1L - ENSG00000120254 [96%]
  
ANTIGEN VIEW
MTHFD1-001
MTHFD1-002
MTHFD1-014
MTHFD1-019

RELEVANT PUBLICATIONS
 
 

Antibody HPA000704

  

Antibody HPA001290

  

Antibody HPA015006

  

Antibody HPA050052

  

1.

 Cancer cell response to anthracyclines effects: mysteries of the hidden proteins associated with these drugs
Tyleckova J et al
Int J Mol Sci 2012;13(12):15536-64
   Elucidating novel hepatitis C virus-host interactions using combined mass spectrometry and functional genomics approaches
Germain MA et al
Mol Cell Proteomics 2014;13(1):184-203
Application: IP, WB 		     
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.