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MTHFD1L

ANTIBODY INFORMATION
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015006   HPA029040   HPA029041   HPA050052   HPA074911  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 MTHFD1 - ENSG00000100714 [100%]
       MTHFD1 - ENSG00000100714 [84%]
    

Released in version

 4   13   13   13   16  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  
Immunocytochemistry

        Supported  
Immunohistochemistry

Approved   Approved   Approved   Approved    
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Standard validation

         Supported  

         

Figure description

         Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.  

Antibody dilution

         1:76  

Literature conformity

         The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Standard validation

 Approved   Approved   Approved   Approved    

         

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 69 cell types		   RNA: detected in 35 tissues
Protein: detected in 72 cell types		   RNA: detected in 35 tissues
Protein: detected in 54 cell types		   RNA: detected in 35 tissues
Protein: detected in 71 cell types		    

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6    

Antibody dilution

 1:250   1:200   1:350   1:55    

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

         

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 110.6, 105.9, 105.8, 101.5, 99.3   105.9, 105.8, 99.3, 36.3, 29.8   105.9, 105.8, 99.3   105.9, 105.8, 101.5, 99.3, 11.5, 10, 6.2   105.9, 105.8, 99.3, 36.3  

Antibody dilution

 1:250   1:250   1:250   1:240   1:250  

PROTEIN ARRAY
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000   1:1950   1:3050  

ANTIGEN INFORMATION
 
 

Antibody HPA015006

  

Antibody HPA029040

  

Antibody HPA029041

  

Antibody HPA050052

  

Antibody HPA074911

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   90   91   25   76  

Antigen sequence

 
AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRL
GIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAP
TEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSK
  
ARDSIVREVIQNSKEVLSLLQEKNPAFKPVLAIIQAGDDNLMQEINQNLA
EEAGLNITHICLPPDSSEAEIIDEILKINEDTRVHGLALQ
  
HFGGLIEEDDVILLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLS
PVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKV
  
FILPISDVRASIGAGFIYPLVGTVS
  
HGSLEAALQCLFQRKGSMTMSIQWKTRQLQSKLHEADIVVLGSPKPEEIP
LTWIQPGTTVLNCSHDFLSGKVGCGS
 

Matching transcripts

 MTHFD1L-003 - ENSP00000356290 [80%]
MTHFD1L-201 - ENSP00000478253 [80%]
MTHFD1L-202 - ENSP00000479539 [80%]
  MTHFD1L-003 - ENSP00000356290 [100%]
MTHFD1L-008 - ENSP00000356277 [100%]
MTHFD1L-012 - ENSP00000356276 [100%]
MTHFD1L-201 - ENSP00000478253 [100%]
MTHFD1L-202 - ENSP00000479539 [93%]
  MTHFD1L-003 - ENSP00000356290 [100%]
MTHFD1L-201 - ENSP00000478253 [100%]
MTHFD1L-202 - ENSP00000479539 [100%]
  MTHFD1L-001 - ENSP00000391022 [96%]
MTHFD1L-003 - ENSP00000356290 [96%]
MTHFD1L-201 - ENSP00000478253 [96%]
MTHFD1L-202 - ENSP00000479539 [96%]
MTHFD1L-002 - ENSP00000399804 [92%]
  MTHFD1L-003 - ENSP00000356290 [100%]
MTHFD1L-008 - ENSP00000356277 [100%]
MTHFD1L-201 - ENSP00000478253 [100%]
MTHFD1L-202 - ENSP00000479539 [100%]
 

Other gene match

 MTHFD1 - ENSG00000100714 [100%]
       MTHFD1 - ENSG00000100714 [84%]
    
ANTIGEN VIEW
MTHFD1L-001
MTHFD1L-002
MTHFD1L-003
MTHFD1L-008
MTHFD1L-012
MTHFD1L-201
MTHFD1L-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.