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TCL1A

ANTIBODY INFORMATION
 
 

Antibody HPA016604

  

Antibody CAB004045

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA016604   4042  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   2  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA016604

  

Antibody CAB004045

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016604

  

Antibody CAB004045

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

Cell line02004006008001,0001,2001,400Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO02004006008001,0001,2001,400Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line0100200300400500600RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO0100200300400500600RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

  
Cell line05001,0001,5002,0002,5003,000Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO05001,0001,5002,0002,5003,000Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line0100200300400500600RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO0100200300400500600RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of lymphoid cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in lymphoid cells outside reaction centra.  

Expression

 RNA: detected in 18 tissues
Protein: detected in 10 cell types		   RNA: detected in 18 tissues
Protein: detected in 21 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016604

  

Antibody CAB004045

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 13.5   13.5, 6.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA016604

  

Antibody CAB004045

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA016604

  

Antibody CAB004045

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 108    

Antigen sequence

 
AECPTLGEAVTDHPDRLWAWEKFVYLDEKQHAWLPLTIEIKDRLQLRVLL
RREDVVLGRPMTPTQIGPSLLPIMWQLYPDGRYRSSDSSFWRLVYHIKID
GVEDMLLE
  

											 
																				

																		

										
											
Matching transcripts

												
																					TCL1A-001 - ENSP00000451506 [100%] 
TCL1A-002 - ENSP00000450496 [100%] 
TCL1A-005 - ENSP00000450701 [100%] 
TCL1A-201 - ENSP00000385036 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TCL1A-001
TCL1A-002
TCL1A-005
TCL1A-201
								

							

						

							
									

			

				
	

	

		
			
				
	

													
															
				
											

									
																				
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA016604

												
										 
																				
											
											
											
Antibody CAB004045

												
										 
																		

																	

										
											
1.

												
																						IGHV3-21 gene usage is associated with high TCL1 expression in chronic lymphocytic leukemia

	Mansouri MR et al

	Eur J Haematol 2010;84(2):109-16

			
											 
																						
											 
																				

																		

										
											
2.

												
																						The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues

	Andersson S et al

	PLoS One 2014;9(12):e115911

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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