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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA016604

 

Antibody CAB004045

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Cell Signaling Technology, Inc
 

Product name

HPA016604   4042  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

   

Released in version

4   2  

References

2    

VALIDATION SUMMARY

 
 

Antibody HPA016604

 

Antibody CAB004045

 
Immunohistochemistry


Validated
 

Validated
 
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA016604

 

Antibody CAB004045

 

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.
 
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.
 

Cell line02004006008001,0001,2001,400Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO02004006008001,0001,2001,400Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line0100200300400500600RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO0100200300400500600RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

 
Cell line05001,0001,5002,0002,5003,000Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO05001,0001,5002,0002,5003,000Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line0100200300400500600RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO0100200300400500600RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

 

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

Supported   Supported  

   

Figure description

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of lymphoid cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in lymphoid cells outside reaction centra.  

Expression

RNA: detected in 18 tissues
Protein: detected in 10 cell types
  RNA: detected in 18 tissues
Protein: detected in 21 cell types
 

Retrieval

HIER pH6   HIER pH6  

Antibody dilution

1:400   1:35  

Literature conformity

Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA016604

 

Antibody CAB004045

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Uncertain

Analysis performed using a standard panel of samples.
No bands detected.
 

   

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

13.5   13.5, 6.2  

Antibody dilution

1:250   1:500  

PROTEIN ARRAY

 
 

Antibody HPA016604

 

Antibody CAB004045

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
   

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:3000    

ANTIGEN INFORMATION

 
 

Antibody HPA016604

 

Antibody CAB004045

 

Antigen

Recombinant protein fragment   Synthetic peptide  

Length (aa)

108    

Antigen sequence

AECPTLGEAVTDHPDRLWAWEKFVYLDEKQHAWLPLTIEIKDRLQLRVLL
RREDVVLGRPMTPTQIGPSLLPIMWQLYPDGRYRSSDSSFWRLVYHIKID
GVEDMLLE
   

Matching transcripts

TCL1A-001 - ENSP00000451506 [100%]
TCL1A-002 - ENSP00000450496 [100%]
TCL1A-005 - ENSP00000450701 [100%]
TCL1A-201 - ENSP00000385036 [100%]
   

Other gene match

   
ANTIGEN VIEW
TCL1A-001
TCL1A-002
TCL1A-005
TCL1A-201

RELEVANT PUBLICATIONS

 
 

Antibody HPA016604

 

Antibody CAB004045

 

1.

IGHV3-21 gene usage is associated with high TCL1 expression in chronic lymphocytic leukemia
Mansouri MR et al
Eur J Haematol 2010;84(2):109-16
   

2.

The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues
Andersson S et al
PLoS One 2014;9(12):e115911
Application: IHC
   
 

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