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Antibody staining overlaps with antibody HPA031157.
Validated
Antibody staining overlaps with antibody HPA003583.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:95
1:30
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003583
Antibody HPA031157
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate to strong positivity in tubular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003583
Antibody HPA031157
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410776)
Target mass (kDa)
61.7, 58.8, 50.7
61.7, 58.8, 50.7, 29.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA003583
Antibody HPA031157
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
