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MMP9

ANTIBODY INFORMATION
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001238   HPA063909   RB-1539   AMAb90804   AMAb90805   AMAb90806  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G   Protein A/G  

Other gene match

            

Released in version

 2   13   1   13   13   13  

References

 2            

VALIDATION SUMMARY
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  
Immunocytochemistry

Approved            
Immunohistochemistry


Validated
  

Validated
   Supported  

Validated
   Supported   Supported  
Western blot

Supported   Supported   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported          

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Standard validation

 Approved            

           

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.            

Antibody dilution

 1:43            

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.            

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     
 

 

      

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		          

 

  
 

          

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA063909 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA001238 across 73 cell types.		          
 

Standard validation

 Supported   Supported   Supported   Supported   Supported   Supported  

           

Figure description

 Immunohistochemical staining of human spleen shows strong positivity in the red pulp.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subset of hematopoietic cells.   Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subset of hematopoietic cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in subset of cells in white pulp and red pulp.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 6 cell types		   RNA: detected in 27 tissues
Protein: detected in 7 cell types		   RNA: detected in 27 tissues
Protein: detected in 25 cell types		   RNA: detected in 27 tissues
Protein: detected in 4 cell types		   RNA: detected in 27 tissues
Protein: detected in 5 cell types		   RNA: detected in 27 tissues
Protein: detected in 6 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:2500   1:1000   1:2000   1:2000   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		  

           

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401553)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401553)		     Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 78.5   78.5   78.5   78.5   78.5   78.5  

Antibody dilution

 1:250   1:250   1:5000   1:1000   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		          

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.          

Antibody dilution

 1:3000   1:4350          

ANTIGEN INFORMATION
 
 

Antibody HPA001238

  

Antibody HPA063909

  

Antibody CAB000348

  

Antibody CAB068199

  

Antibody CAB068200

  

Antibody CAB068201

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 146   74          

Antigen sequence

 
PRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPA
LLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHN
ITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADI
  
WRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRV
SSRSELNQVDQVGYVTYDILQCPE
  

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					MMP9-001 - ENSP00000361405 [100%] 

											 
																						MMP9-001 - ENSP00000361405 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MMP9-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001238

												
										 
																				
											
											
											
Antibody HPA063909

												
										 
																				
											
											
											
Antibody CAB000348

												
										 
																				
											
											
											
Antibody CAB068199

												
										 
																				
											
											
											
Antibody CAB068200

												
										 
																				
											
											
											
Antibody CAB068201

												
										 
																		

																	

										
											
1.

												
																						Histone deacetylase (HDAC) 10 suppresses cervical cancer metastasis through inhibition of matrix metalloproteinase (MMP) 2 and 9 expression

	Song C et al

	J Biol Chem 2013;288(39):28021-33

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Investigation of potential early Histologic markers of pediatric inflammatory bowel disease

	Bass JA et al

	BMC Gastroenterol 2015;15:129

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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