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NDUFAF5

ANTIBODY INFORMATION
 
 

Antibody HPA051184

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051184  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA051184

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051184

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human nasopharynx shows strong membranous positivity in respiratory epithelial cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:150  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051184

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.9, 36.1  

Antibody dilution

 1:490  

PROTEIN ARRAY
 
 

Antibody HPA051184

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3950  

ANTIGEN INFORMATION
 
 

Antibody HPA051184

  

Antigen

 Recombinant protein fragment  

Length (aa)

 94  

Antigen sequence

 
VNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFS
PHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMED
 

Matching transcripts

 NDUFAF5-006 - ENSP00000367346 [100%]
NDUFAF5-008 - ENSP00000420497 [100%]
 

Other gene match

  
ANTIGEN VIEW
NDUFAF5-006
NDUFAF5-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.