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Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
Antibody dilution
1:169
1:19
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008003
Antibody HPA029735
Antibody CAB000329
Antibody CAB019308
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 30 tissues Protein: detected in 70 cell types
RNA: detected in 30 tissues Protein: detected in 69 cell types
RNA: detected in 30 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:75
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008003
Antibody HPA029735
Antibody CAB000329
Antibody CAB019308
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417432)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
46.9
46.9
46.9
46.9
Antibody dilution
1:250
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA008003
Antibody HPA029735
Antibody CAB000329
Antibody CAB019308
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
