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SLC32A1

ANTIBODY INFORMATION
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA058859   HPA059985   V5764  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   7  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA059985 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA058859 across 75 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human retina shows strong cytoplasmic positivity in inner plexiform layer.   Immunohistochemical staining of human retina shows strong cytoplasmic positivity in inner plexiform layer.   Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in inner plexiform layer.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 1 cell types		   RNA: detected in 1 tissues
Protein: detected in 1 cell types		   RNA: detected in 1 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:1200   1:1200  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 57.4   57.4   57.4  

Antibody dilution

 1:200   1:370   1:500  

PROTEIN ARRAY
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2350   1:4500    

ANTIGEN INFORMATION
 
 

Antibody HPA058859

  

Antibody HPA059985

  

Antibody CAB037156

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 91   42    

Antigen sequence

 
ATSVSNKSQAKMSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDI
LKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQVG
  
LIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVN
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC32A1-001 - ENSP00000217420 [100%] 

											 
																						SLC32A1-001 - ENSP00000217420 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC32A1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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