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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:196
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039890
Antibody HPA052946
Standard validation
Approved
Figure description
Immunohistochemical staining of human rectum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039890
Antibody HPA052946
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
102.6, 101.9, 98.4, 65.5, 18.5
102.6, 101.9, 98.4, 65.5
Antibody dilution
1:250
1:980
PROTEIN ARRAY
Antibody HPA039890
Antibody HPA052946
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
