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ASB9

ANTIBODY INFORMATION
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA003014   HPA003060   H00140462-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   3   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  
Immunocytochemistry

Uncertain   Uncertain    
Immunohistochemistry

Supported   Supported  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.    

Antibody dilution

 1:31   1:40    

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong nuclear and moderate cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong positivity in seminiferous ducts.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 21 cell types		   RNA: detected in 31 tissues
Protein: detected in 15 cell types		   RNA: detected in 31 tissues
Protein: detected in 47 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:250   1:700  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.9, 27.9, 26.8   31.9, 27.9, 26.8, 22.5   31.9, 27.9, 26.8, 22.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003014

  

Antibody HPA003060

  

Antibody CAB004997

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 105   129    

Antigen sequence

 
GMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLI
SQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPL
FNACV
  
SVQPESDLASPIHEAARRGHVECVNSLIAYGGNIDHKISHLGTPLYLACE
NQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQ
AKNAEGKRPVELVPPESPLAQLFLEREGA
  

											 
																				

																		

										
											
Matching transcripts

												
																					ASB9-001 - ENSP00000369855 [100%] 
ASB9-002 - ENSP00000369852 [100%] 
ASB9-003 - ENSP00000369850 [100%] 
ASB9-201 - ENSP00000438943 [100%] 

											 
																						ASB9-002 - ENSP00000369852 [100%] 
ASB9-201 - ENSP00000438943 [100%] 
ASB9-001 - ENSP00000369855 [99%] 
ASB9-008 - ENSP00000473542 [98%] 
ASB9-003 - ENSP00000369850 [92%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ASB9-001
ASB9-002
ASB9-003
ASB9-008
ASB9-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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