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EMD

ANTIBODY INFORMATION
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Novocastra
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000609   SC-15378   NCL-EMERIN   AMAb90560  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Not known   Protein A/G  

Other gene match

        

Released in version

 1   1   1   12  

References

 2        

VALIDATION SUMMARY
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  
Immunocytochemistry

Supported   Supported   Supported    
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain   Supported  
Protein array

Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Standard validation

 Supported   Supported   Supported    

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.    

Antibody dilution

 1:100   1:2000   1:20    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.   Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.   Immunohistochemical staining of human kidney shows distinct of nuclear membranes positivity in renal cells.   Immunohistochemical staining of human testis shows nuclear and membranous positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:2000   1:80   1:5000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		       Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 29, 24.9   29, 24.9   29, 24.9   29, 24.9  

Antibody dilution

 1:500   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:1000        

ANTIGEN INFORMATION
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

Antigen

 Recombinant protein fragment   Recombinant protein   Not known   Recombinant protein  

Length (aa)

 112        

Antigen sequence

 
ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTY
GEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMY
GRDSACQSITHY
       

Matching transcripts

 EMD-001 - ENSP00000358857 [99%]
EMD-010 - ENSP00000358850 [93%]
       

Other gene match

        
ANTIGEN VIEW
EMD-001
EMD-010

RELEVANT PUBLICATIONS
 
 

Antibody HPA000609

  

Antibody CAB001545

  

Antibody CAB002029

  

Antibody CAB062552

  

1.

 Impact of genomic stability on protein expression in endometrioid endometrial cancer
Lomnytska MI et al
Br J Cancer 2012;106(7):1297-305
        

2.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
        
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.