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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:88
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040810
Antibody HPA042037
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 24 tissues Protein: detected in 63 cell types
RNA: detected in 24 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:70
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040810
Antibody HPA042037
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
114.8, 108.9, 108.1, 87.8, 80.1, 75.5
114.8, 108.9, 108.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040810
Antibody HPA042037
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
