SLC6A2 - Antibodies - The Human Protein Atlas

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SLC6A2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA004057

Antibody CAB078195

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA004057

AMAb91116

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA004057

Antibody CAB078195

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA004057

Antibody CAB078195

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.

Antibody dilution

1:43

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA004057

Antibody CAB078195

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows positivity in neuronal processes.

Expression

RNA: detected in 7 tissues
Protein: detected in 5 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Standard validation

Supported

Figure description

Noradrenergic fibers arising from the locus coeruleas innervating the entire brain.

WESTERN BLOT

Antibody HPA004057

Antibody CAB078195

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

70.5, 69.3, 67.7, 64.3, 62.9, 57.4, 33.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA004057

Antibody CAB078195

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA004057

Antibody CAB078195

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

SLYYLFSSFTLNLPWTDCGHTWNSPNCTDPKLLNGSVLGNHTKYSKYKFT
PAAEFYERGVLHLHESSGIHDI

Matching transcripts

SLC6A2-001 - ENSP00000369237 [100%]
SLC6A2-003 - ENSP00000394956 [100%]
SLC6A2-004 - ENSP00000457473 [100%]
SLC6A2-005 - ENSP00000457375 [100%]
SLC6A2-006 - ENSP00000456210 [100%]
SLC6A2-007 - ENSP00000454439 [100%]
SLC6A2-009 - ENSP00000456377 [100%]
SLC6A2-201 - ENSP00000219833 [100%]

Other gene match

ANTIGEN VIEW

SLC6A2-001	SLC6A2-003	SLC6A2-004	SLC6A2-005	SLC6A2-006	SLC6A2-007	SLC6A2-009	SLC6A2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.