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AAGAB

ANTIBODY INFORMATION
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039371   HPA040174  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

   2  

VALIDATION SUMMARY
 
 

Antibody HPA039371

  

Antibody HPA040174

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.  

Antibody dilution

   1:33  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA040174 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA039371 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.6, 22.7   34.6, 22.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039371

  

Antibody HPA040174

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 89   77  

Antigen sequence

 
KAQEWCIKHGFELVELSPEELPEEDDDFPESTGVKRIVQALNANVWSNVV
MKNDRNQGFSLLNSLTGTNHSIGSADPCHPEQPHLPAAD
  
GGASNTTDAQVDSIVDPMLDLDIQELASLTTGGGDVENFERLFSKLKEMK
DKAATLPHEQRKVHAEKVAKAFWMAIG
 

Matching transcripts

 AAGAB-001 - ENSP00000261880 [100%]
AAGAB-002 - ENSP00000440735 [100%]
AAGAB-003 - ENSP00000453263 [100%]
  AAGAB-001 - ENSP00000261880 [100%]
AAGAB-002 - ENSP00000440735 [100%]
AAGAB-003 - ENSP00000453263 [100%]
 

Other gene match

    
ANTIGEN VIEW
AAGAB-001
AAGAB-002
AAGAB-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA039371

  

Antibody HPA040174

  

1.

   Haploinsufficiency for AAGAB causes clinically heterogeneous forms of punctate palmoplantar keratoderma
Pohler E et al
Nat Genet 2012;44(11):1272-6
  

2.

   Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.